基于响应面法的艾草总黄酮提取工艺优化Optimization of extraction process of total flavonoids from Artemisia argyi based on response surface methodology
王丽焕,郭文娟,钱晓明
摘要(Abstract):
为提高艾草纤维的抗菌效果,应用超声波辅助提取技术从艾草中提取总黄酮,采用单因素实验考察提取时间、提取温度、料液比、乙醇体积分数等条件对艾草总黄酮提取率的影响,采用响应曲面分析法,研究艾草总黄酮的最佳提取工艺。结果表明:乙醇体积分数、料液比及提取温度对总黄酮提取率的影响较大,影响大小顺序为料液比>乙醇体积分数>提取温度,艾草总黄酮提取率随着料液比和提取温度的增加而增大,随着乙醇体积分数的增加先增大后降低;超声波辅助提取艾草总黄酮的最佳工艺条件为艾草粉碎100目、乙醇体积分数50%、提取温度80℃、提取时间20 min、料液比1∶40(g/mL)、提取次数3次,在此条件下艾草总黄酮的提取率可达167.02 mg/g。
关键词(KeyWords): 艾草;总黄酮;超声波辅助;响应曲面分析法;提取率
基金项目(Foundation): 天津市自然科学基金资助项目(20JCQNJC00250)
作者(Author): 王丽焕,郭文娟,钱晓明
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