曹占平,宋晓绪,范云双,王尚尚,苏建东,刘恩华

• 1:天津工业大学环境与化学工程学院
• 2:天津工业大学分离膜与膜过程国家重点实验室/分离膜科学与技术国家级国际合作研究中心
• 3:天津瑞普生物技术股份有限公司

摘要(Abstract)：

为提高转移因子的生产效率,采用管式微滤+超滤装置代替透析袋的工序提取猪脾脏中的转移因子,研究分离膜工艺中猪脾脏均质液的稀释方法、冻融次数及膜清洗恢复情况.实验结果表明:脾脏均质液冻融3次,采用纯水1∶1(体积比)稀释,在过滤过程中不断添加纯水以降低操作温度并提高过滤效率.微滤和超滤操作约3 h完成,操作温度始终低于37℃,更适宜转移因子的生产,超滤透过液中多肽、核糖的含量也符合半成品的要求.改进后的工艺操作简单,成本低,生产周期短,可适用于大规模工业生产.

关键词(KeyWords)： 分离膜;转移因子;管式膜;微滤;超滤

Abstract:

Keywords:

基金项目(Foundation): 天津市海洋经济创新发展区域示范项目(CXSF2014-24)

作者(Author): 曹占平,宋晓绪,范云双,王尚尚,苏建东,刘恩华

参考文献(References)：

• [1]KLESIUS P H,FUDENBERG H H.Bovine transfer factor:In vivo,transfer of cell-mediated immunity to cattle with alcohol precipitates[J].Clinical Immunology&Immunopathology,1977,8(2):238-246.
• [2]JAMKAR A V.Immunotherapy of advanced carcinoma maxilla with immunosensitised porcine mesentric lymph node cells with immunospecific xenogenic lymphnode cell transfer factor[J].Indian Journal of Cancer,1980,17(1):25-30.
• [3]KLESIUS P H,GIAMBRONE J J.Adoptive transfer of delayed hypersensitivity and protective immunity to eimeriatenella with chicken-derived transfer factor[J].Poultry Science,1984,63(7):1333-1337.
• [4]SIMON M R,JR S J,FREIER D,et al.Tuberculin-specific transfer factor in dogs[J].Infection&Immunity,1977,18(1):73-77.
• [5]张广英,徐可利,王玉茂,等.兽用转移因子的应用[J].中国畜禽传染病,1991,60(5):68-69.ZHANG G Y,XU K L,WANG Y M,et al.The application of the transfer factor in veterinary use[J].Chinese Animal and Poultry Infectious Diseases,1991,60(5):68-69(in Chinese).
• [6]MADDISON S E,HICKLIN M D,CONWAY B P,et al.Transfer factor:Delayed hypersensitivity to Schistoso mamansoni and tuberculin in Macacamulatta[J].Science,1972,178(4062):757-759.
• [7]MISHRA S S,JAISWAL T N.Transfer of delayed hypersensitivity by dialysable lymphocyte extract from Listeriamonocytogenes sensitized rabbits[J].Indian Vet,1992,69(1):1-4.
• [8]成杰,栾业俊,仲亚娜,等.转移因子的研究进展[J].山东畜牧兽医,2017,38(8):55-57.CHENG J,LUAN Y J,ZHONG Y N,et al.Advances in transfer factors[J].Shandong Animal Husbandry and Veterinary Medicine,2017,38(8):55-57(in Chinese).
• [9]顾平,陈德有.猪脾转移因子生产工艺的改进[J].中国生化药物杂志,2001,22(2):94-95.GU P,CHEN D Y.Improvement of pig spleen transfer factor production process[J].Chinese Journal of Biochemical Medicine,2001,22(2):94-95(in Chinese).
• [10]张立武,万星,孔建平,等.猪脾转移因子的规模化生产工艺研究[J].畜牧与兽医,2011,43(5):61-63.ZHANG L W,WAN X,KONG J P,et al.Study on large scale production process of pig spleen transfer factor[J].Journal of Animal Husbandry and Veterinary Medicine,2011,43(5):61-63(in Chinese).
• [11]朱天新,任晚琼,袁彩君.猪脾转移因子生产方法的比较[J].中国生化药物杂志,2005,26(1):44-45.ZHU T X,REN W Q,YUAN C J.Comparison of production methods of pig spleen transfer factor[J].Chinese Journal of Biochemical Pharmaceutics,2005,26(1):44-45(in Chinese).
• [12]刘达玉,钟世荣.管式膜超滤生黄酒的研究[J].食品科学,2004,25(3):110-112.LIU D Y,ZHONG S R.Study on ultrafiltration of rice film by tube film[J].Food Science,2004,25(3):110-112(in Chinese).
• [13]王尚尚,苏建东,杨燚,等.一种猪脾转移因子纯化方法:中国,CN103724419A[P].2014-04-16.WANG S S,SU J D,YANG Y,et al.A method for purification of porcine splenic transfer factor:China,CN103724419A[P].2014-04-16(in Chinese).
• [14]王甜,苏建东,王尚尚,等.一种猪脾转移因子制备方法:中国,CN103705539A[P].2014-04-09.WANG T,SU J D,WANG S S,et al.A method for preparing pig spleen and transfer factor:China,CN103705539A[P].2014-04-09(in Chinese).
• [15]王彦宏,冷南,王树宽,等.不同温度贮存的转移因子对淋巴细胞增殖反应的影响[J].第四军医大学学报,1996,17(3):240.WANG Y H,LENG N,WANG S K,et al.Effects of different temperature storage transfer factors on lymphocyte proliferation response[J].Journal of the Fourth Military Medical University,1996,17(3):240(in Chinese).