何洋,江鹏,赵义平,陈莉

• 1:天津工业大学材料科学与工程学院
• 2:天津工业大学省部共建分离膜与膜过程国家重点实验室

摘要(Abstract)：

为实现对染料废水的高效降解,以聚偏氟乙烯(PVDF)分离膜为载体,利用原子转移自由基聚合(ATRP)引发聚甲基丙烯酸缩水甘油酯(PMGA)接枝在膜表面,通过环氧基与伯胺基的反应,制备出固定化漆酶的分离膜,以实现对废水处理截留过程中高效的染料废水降解。结果表明:漆酶固定化后使膜材料具有高效的催化效率,在4 h后均能达到60%以上,最高可至97.2%±1.4%。同时进一步地利用聚乙烯亚胺(PEI)在固定漆酶的PGMA刷间形成交联网络,有益于维持酶构象稳定性从而保留较高的酶活性,而适当的交联浓度能最大程度的提高膜的降解能力,其中0.5 mmol/L PEI交联改性的漆酶固定化膜表现出最佳的降解能力。

关键词(KeyWords)： 聚偏氟乙烯膜;漆酶;原子转移自由基聚合;固定化;染料废水降解

Abstract:

Keywords:

基金项目(Foundation): 国家自然科学基金资助项目（51973158）;; 天津市自然科学基金资助项目（18JCZDJC99500）

作者(Author): 何洋,江鹏,赵义平,陈莉

参考文献(References)：

• [1] RODR魱GUEZ COUTO S,TOCA HERRERA J L. Industrial and biotechnological applications of laccases:A review[J].Biotechnology Advances,2006,24(5):500-513.
• [2] WONG Y,YU J. Laccase-catalyzed decolorization of synthetic dyes[J]. Water Research,1999,33(16):3512-3520.
• [3] MODARESSI K,TAYLOR K E,BEWTRA J K,et al. Laccase-catalyzed removal of bisphenol-A from water:Protective effect of PEG on enzyme activity[J]. Water Research,2005,39(18):4309-4316.
• [4] FREIRE R S,DURN N,KUBOTA L T. Effects of fungal laccase immobilization procedures for the development of a biosensor for phenol compounds[J]. Talanta,2001,54(4):681-686.
• [5] PEZZELLA C,GIACOBBE S,GIACOBELLI V G,et al. Green routes towards industrial textile dyeing:A laccase based approach[J]. Journal of Molecular Catalysis B:Enzymatic,2016,134:274-279.
• [6]张寒飞.磁性固定化漆酶的制备及其应用研究[D].雅安：四川农业大学，2005.ZHANG H F. Preparation and application of magnetic immobilized laccase[D]. Ya′an:Sichuan Agricultural University,2005(in Chinese).
• [7] AYYARU S,AHN Y. Application of sulfonic acid group functionalized graphene oxide to improve hydrophilicity,permeability,and antifouling of PVDF nanocomposite ultrafiltration membranes[J]. Journal of Membrane Science,2017,525:210-219.
• [8] BARREDO-DAMAS S,ALCAINA-MIRANDA M,BESPIA A,et al. Ceramic membrane behavior in textile wastewater ultrafiltration[J]. Desalination,2010,250(2):623-628.
• [9] NGAH W W S,TEONG L C,HANAFIAH M A K M. Adsorption of dyes and heavy metal ions by chitosan composites:A review[J]. Carbohydrate Polymers,2011,83(4):1446-1456.
• [10] YAGUB M T,SEN T K,AFROZE S,et al. Dye and its removal from aqueous solution by adsorption:A review[J]. Advances in Colloid and Interface Science,2014,209:172-184.
• [11] EDWARDS W,LEUKES W D,ROSE P D,et al. Immobilization of polyphenol oxidase on chitosan-coated polysulphone capillary membranes for improved phenolic effluent bioremediation[J]. Enzyme and Microbial Technology,1999,25(8/9):769-773.
• [12] BOWEN W R,CASSEY B,JONES P,et al. Modelling the performance of membrane nanofiltration:Application to an industrially relevant separation[J]. Journal of Membrane Science,2004,242(1/2):211-220.
• [13]杨振生.热致相分离法iPP中空纤维微孔膜及其形态结构研究[D].天津：天津大学，2005.YANG Z S. Studies on preparation and morphology controlling of iPP hollow fiber microporous membrane via thermally induced phase separation[D]. Tianjin:Tianjin University,2005(in Chinese).
• [14] LEI C H,SHIN Y,LIU J,et al. Entrapping enzyme in a functionalized nanoporous support[J]. Journal of the American Chemical Society,2002,124(38):11242-11243.
• [15] YANG X,HUA L,GONG H,et al. Covalent immobilization of an enzyme(glucose oxidase)onto a carbon sol-gel silicate composite surface as a biosensing platform[J]. Analytica Chimica Acta,2003,478(1):67-75.
• [16] RONG J,ZHANG T,QIU F X,et al. Preparation of efficient,stable,and reusable laccase-Cu3(PO4)2hybrid microspheres based on copper foil for decoloration of Congo red[J]. ACS Sustainable Chemistry&Engineering,2017,5(5):4468-4477.
• [17] HAN Y,SONG S J,LU Y,et al. A method to modify PVDF microfiltration membrane via ATRP with low-temperature plasma pretreatment[J]. Applied Surface Science,2016,379:474-479.
• [18] GOOCH J W. Michaelis-Menten Equation[M]//Encyclopedia of Genetics,Genomics,Proteomics and Informatics. Dordrecht:Springer Netherlands. 2008:1202.
• [19] WANG C Z,ZHAO S,WEI Y M. Hydrophilic modification of microporous polysulfone membrane via surface-initiated atom transfer radical polymerization and hydrolysis of poly(glycidylmethacrylate)[J]. Chinese Journal of Chemistry,2012,30(10):2473-2482.
• [20] ZHAO S,WANG Z. A loose nano-filtration membrane prepared by coating HPAN UF membrane with modified PEI for dye reuse and desalination[J]. Journal of Membrane Science,2017,524:214-224.