辐射接枝聚丙烯纤维制备蛋白质分子印迹聚合物水凝胶Preparation of protein molecular imprinted polymer hydrogel by radiation grafting of polypropylene
申磊,魏俊富,赵孔银
摘要(Abstract)：
以聚丙烯(PP)纤维无纺布为基体,以丙烯酰胺为功能单体,以N,N′-亚甲基双丙烯酰胺为交联剂,采用辐射接枝的方法制备了纤维基牛血清白蛋白(BSA)分子印迹聚合物水凝胶,并用红外光谱仪对接枝聚合物进行了表征.结果表明,聚丙烯酰胺水凝胶被成功接枝到PP纤维上;印迹和非印迹聚合物的动力学和热力学吸附数据表明,印迹聚合物对蛋白质的吸附量明显大于非印迹聚合物的吸附量.接枝率随单体浓度的增加而增大,在单体质量分数为20%时,接枝率达到稳定值;接枝率随交联剂浓度的增加先增大后减小,当交联剂质量分数为2%时,接枝率达到最大值.
关键词(KeyWords)： 聚丙烯无纺布;蛋白质;分子印迹;水凝胶;辐射接枝
基金项目(Foundation): 天津市高等学校科技发展基金(2006ZD40);; 天津市科技发展计划项目(06TXTJJC14400);; 教育部高校博士点基金(20050058001)
作者(Author): 申磊,魏俊富,赵孔银
参考文献(References)：
- [1]SHI H,TSAI W-B,FERRARI S,et al.Template imprinted nanostructural surfaces for protein recognition[J].Nature,1999,398:593-597.
- [2]CHEN Zhiyong,HUA Zhendong,LI Xu,et al.Protein-responsive imprinted polymers with specific shrink ing and Adsorption[J].Journal of Molecular Recognition,2008,21(1):71-77.
- [3]HJERTEN S J,LIAO J L,NAKAZATO K.Gels mimicking antibodies in their selective recognition of protein[J].Chromat-ography,1997,44:227-234.
- [4]GUO Tian ying,XIA Yong qing,WANG Jin,et al.Chitosan beads as molecularly imprinted polymer matrix for selective seperation of proteins[J].Biomaterials,2005,26:5737-5745.
- [5]FU Guoqi,ZHAO Jichao,YU Hao,et al.Bovine serum albumin-imprinted polymer gels prepared by graft copolymer-ization of acrylamide on chitosan[J].Reactive&Functional Polymers,2007,67:442-450.
- [6]TURIEL E,TADEO J L,MARTIN-ESTEBAN A.Molecularly imprinted polymeric fibers for Solid-Phase microextraction[J].Analytical Chemistry,2007,79:3099-3104.